Programmed factor binding to simian virus 40 GC-box replication and transcription control sequences
نویسندگان
چکیده
منابع مشابه
Relationship of replication and transcription of Simian Virus 40 DNA.
RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was...
متن کاملSpecific stimulation of simian virus 40 late transcription in vitro by a cellular factor binding the simian virus 40 21-base-pair repeat promoter element.
We have identified a cellular transcription factor from uninfected HeLa cells that stimulates the simian virus 40 (SV40) late mode of transcription and specifically binds the SV40 21-base-pair repeat promoter element. In particular, the late SV40 transcription factor (LSF) stimulates transcription at initiation sites L325 and L264 of the SV40 late promoter, which are the major transcription sit...
متن کاملSimian Virus 40 Activates ATR- p53 Signaling To Override Cell Cycle and DNA Replication Control
During infection, simian virus 40 (SV40) attempts to take hold of the cell, while the host responds with various defense systems, including the ataxia-telangiectasia mutated/ATM-Rad3 related (ATM/ATR)-mediated DNA damage response pathways. Here we show that upon viral infection, ATR directly activates the p53 isoform p53, leading to upregulation of the Cdk inhibitor p21 and downregulation of cy...
متن کاملOrigin and direction of simian virus 40 deoxyribonucleic acid replication.
Double-branched, circular, replicating deoxyribonucleic acid (DNA) molecules of simian virus 40 (SV40) have been cleaved by the R(1) restriction endonuclease from Escherichia coli. This enzyme introduces one double-strand break in SV40 DNA, at a specific site. The site of cleavage in the replicating molecules was used in this study to position the origin and the two branch points. Radioactively...
متن کاملMeasurement of the binding of transcription factor Sp1 to a single GC box recognition sequence.
The equilibrium constant was determined for the binding of the transcription factor Sp1 to a single consensus GC box DNA recognition site, (5'-GGGGCGGGGC-3'). For these experiments, single copies of the recognition site were synthesized and cloned in a standard plasmid background. Binding was measured either by a footprinting assay modified so that the binding reaction was at equilibrium, or by...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1990
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.64.1.347-353.1990